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In Vitro Antibacterial Activity of Galla Chinensis Combined with Different Antibacterial Drugs against Carbapenem-Resistant E.Coli

Authors

  • Zhizhi Xie Email Department of Laboratory Medicine, The Third Affliated Hospital of Sun Yat-sen University
  • Changzhi Xu Department of Laboratory Medicine, The Third Affliated Hospital of Sun Yat-sen University
  • Yanhua Yi Department of Emergency, The Third Affliated Hospital of Sun Yat-sen University
  • Donglin Zhu Department of Laboratory Medicine, The Third Affliated Hospital of Sun Yat-sen University
  • Yun Xi Department of Laboratory Medicine, The Third Affliated Hospital of Sun Yat-sen University

DOI:

https://doi.org/10.30564/jams.v2i4.1229
Received: 18 September 2019 | Published Online: 5 November 2019

Abstract

Objective: To evaluate the antibacterial effects of meropenem and levofloxacin respectively combined with Galla chinensis on carbapenem-resistant Escherichia coli in vitro. Methods： The protocol was designed with checkerboard method and the carbapenem-resistant E.coli was isolated in our hospital. The minimum inhibitory concentrations(MICs) of G. chinensis alone and combined with 2 antimicrobial agents against carbapenem-resistant E.coli were determined by broth dilution method and the fractional inhibitory concentration index (FICI) was calculated according to MICs results. Result： the combined use of G. chinensis and meropenem (or levofloxacin) significantly decreased both MIC50 and MIC90; After the combination of G. chinensis and meropenem, the synergistic effect was 86.7%, and the additive effect was 13.3%, no irrelevant and antagonistic effects. After combined use of G. chinensis and levofloxacin, the synergistic effect was 66.7%, and the additive effect was 33.3%. No irrelevant and antagonistic effects. Conclusion： Galla chinensis combined with meropenem or levofloxacin has synergistic and additive antibacterial effect, with certain combined antibacterial activity.

Keywords:

Galla chinensis, Carbapenem resistance, Escherichia coli, combination therapy

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2019 Vol.2 No.4

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Copyright © 2019 Zhizhi Xie, Changzhi Xu, Yanhua Yi, Donglin Zhu, Yun Xi

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